Isolasi dan Karakterisasi Senyawa Triterpenoid dari Ekstrak Etil Asetat Daun Salam (Syzygium polyanthum (Wight) Walp.) serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test)
DOI:
https://doi.org/10.25077/jku.12.1.7-13.2023Keywords:
syzgium polyanthum (wight) Walp, triterpenoids, cytotoxicAbstract
The bay plant (Syzygium polyanthum (Wight) Walp.) is a member of the myrtaceae family. The most widely used part of the bay plant is the bay leaf. The leaves of this plant are often used as traditional medicine to treat gout, diarrhea, diabetes, and high blood pressure. This study aims to isolate and determine the characteristics of triterpenoid compounds from ethyl acetate extract of bay leaves. Isolation was carried out by column chromatography method with SGP (Step Gradient Polarity) elution system, then the isolated compound was purified by recrystallization method and obtained in the form of white crystals weighing 0.0188 g. TLC results showed a single purple spot after being treated with Liebermann Buschard reagent with a melting point range of 137-139 oC. The results of the UV spectrum data showed no conjugated double bonds and the results of the FTIR spectrum data showed the presence of hydroxyl groups, C-O alcohols, C-H alkanes, C=C alkenes, geminal dimethyl which are characteristic of triterpenoid compounds. Cytotoxic activity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method. Ethyl acetate extract and methanol extract had strong cytotoxic activity with LC50 values 316.23 mg/L and 234.42 mg/L respectively, while the hexane extract was weak with an LC50 value of 1148.15 mg/L.
References
Novira, P. P., Febrina, E.(2018). Tinjauan Aktivitas Farmakologi Ekstrak Daun Salam (Syzygiu polyanthum
Silalahi, M.(2017). Syzygium polyanthum (Wight) Walp. (Botani, Metabolit Sekunder dan Pemanfaatan). JurnalDinamika Pendidikan, 10(1), 187–202.
Harismah, K.; Chusniatun.(2016). Pemanfaatan Daun Salam (Eugenia polyantha) Sebagai Obat Herbal Dan Rempah Penyedap Makanan.War. LPM 2016, 19, 110– 118.
Rizki, M. I.; Hariandja, E. M.(2016). Aktivitas Farmakologis, Senyawa Aktif, dan Mekanisme Kerja Daun Salam (Syzygium polyanthum). Universitas Lambung Mangkurat,Banjarbaru .
Kilis, T. N. I. M.; Karauwan, F. A.; Sambou, C. N.; Lengkey, Y.K.(2020).Formulasi Sediaan Salep Ekstrak Daun Salam Syzygium polyanthum Sebagai Antibakteri Staphylococcus aureus. Jurnal Biofarmasetikal Tropis , 3(1), 46-53.
Ayni, R.(2020). Identifikasi Metabolit sekunder Dan Uji Antibakteri Dari Daun Salam (Syzygium polyanthum (Wight) Walp).Skripsi. Fakultas Matematika Dan Ilmu Pengatahuan Alam, Universitas Andalas, Padang .
Nurhamidah, H Nurdin, Y Manjang, A Dharma, and Suryati, (2018). Cytotoxic activity assay from leaves and fruit extracts of Ficusaurata (Miq.) using brine shrimp lethality test method. IOP Conf Series: Journal of Physics: Conf. Series 1116 (2018) 042028
Carballo, J. L. I.; Inda, Z. L. H. and Perez (2002). A Comparison between Two Brine Shrimp Assay to Detect in Vitro Cytotoxicity in Marine Natural Product. BMC Biotecnology. 2002, 2, 17, 1-5.
Mayanti,T. Aneu Wahyuni, Indri Indriyani, Darwati, Tati Herlina, Unang Supratman. (2017). Senyawa-Senyawa Aromatik dari Ekstrak Daun dan Kulit Batang Dysoxylum parasiticum Serta Toksisitasnya Terhadap Artemia salina, Chimica et Natura Acta Vol. 5 No. 1: 26-30
Santoni, A. Sabariah, Efdi, M.(2015). Isolasi Dan Elusidasi Struktur Senyawa Triterpenoid Dari Kulit Batang Ambacang (Mangifera Foetida L.) Serta Uji Brine Shrimp Letality Test (BSLT), Jurnal Riset Kimia Vol. 9 No.1
Ibrahim, S. (1998). Teknik Laboratorium Kimia Organik. Pasca Sarjana Universitas Andalas .
Efdi, M.; Syafrizayanti.; Sari, D. K.(2016). Isolasi dan Karakterisasi Terpenoid Serta UjiAntioksidan dari Ekstrak Kulit Batang Shorea singkawan. Chempublish .Journal 2016, 1, 64.
Suzery, M.; Gultom, M. C. B.(2013). Senyawa Hiptolida Dan Pektinolida Dalam Fraksi Diklorometana Dari Daun Hyptis Pectinata Poit. Journal Sains dan Matematika , 21 (32), 1–4.
Silverstein, RM.; Bessler, G.C.; Moril.(1989). Spektrometric Identification of OrganicCompound (Penyidikan Spektroskopi Senyawa Organik). Penerbit Erlangga, Jakarta .
Pretsch E, Buhlmann P, Badertscher M.(2009) Structure Determination of Organic Compounds Tables of Spectral Data .Fourth, Revised and Enlarged Edition. Springer- Verlag Berlin Heidelberg . 13-404. 16(Wight.) Walp) , Suplemen Volume 16 Nomor 2 hal 288-297.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Jurnal Kimia Unand
This work is licensed under a Creative Commons Attribution 4.0 International License.
Please find the rights and licenses in Jurnal Kimia Unand (J. Kim. Unand). Authors who publish with J. Kim. Unand agree to the following policies. No specific document sign-off is required.
1. License
The use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License.Â
2. Author(s)' Warranties
The author warrants that the article is original, written by the stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons license, the journal permits users to copy, distribute, and display the material. Users will also need to attribute authors and J. Kim. Unand on distributing works in the journal and other media of publications.
4. Rights of Authors
Authors retain all their rights to the published works, such as (but not limited to) the following rights;
- Copyright and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in own future works, including lectures and books,
- The right to reproduce the article for own purposes,
- The right to self-archive the article,
- The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
5. Co-Authorship
Suppose more than one author jointly prepared the article. In that case, any author submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf; and agrees to inform his/her co-authors of the terms of this policy. J. Kim. Unand will not be held liable for anything arising due to the author(s) internal dispute. J. Kim. Unand will only communicate with the corresponding author.
6. Royalties
By submitting the articles, the authors agreed that no fees are payable from J. Kim. Unand.
7. Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, J. Kim. Unand cannot guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.
8. Miscellaneous
J. Kim. Unand will publish the article (or have it published) in the journal if the article’s editorial process is completed and J. Kim. Unand has become obligated to have the article published. The author acknowledges that the article may be published so that it will be publicly accessible, and such access will be free of charge for the readers.
