Isolasi Senyawa Metabolit Sekunder Dari Fraksi Aktif Antibakteri Pada Tumbuhan Sirih Merah (Piper porphyrophyllum)
DOI:
https://doi.org/10.25077/jku.12.2.1-7.2023Keywords:
Piper porphyrophyllum; Isolation; Antibacteria; UV-Visible; TriterpenoidsAbstract
The red betel plant (Piper porphyrophyllum) belongs to the Piperaceae family which is traditionally used to treat various diseases, including headaches, bone pain, chest tightness, leprosy, stomach ache, as well as for various other diseases such as skin diseases and diabetes. In this study, the antibacterial activity was tested for the hexane, ethyl acetate and methanol extracts of red betel nut using the disc diffusion method and the isolation of secondary metabolites from the hexane fraction of red betel nut using column chromatography technique. Determination of the antibacterial activity of the three fractions showed that the methanol extract had better antibacterial activity compared to the hexane extract and ethyl acetate extract. UV spectrum analysis showed 3 bands with absorption of 282 nm in band I, 274.80 nm in band II and 224.80 nm in band III which means that they show characteristics for triterpenoid group compounds. FTIR analysis showed the presence of C-H, CH2, C=O, C-O alcohol and geminal dimethyl functional groups in the isolated compounds.
References
Suharno, R.H.R. Tanjung, S. Sufaati, V. Agustini, Wati (Piper methysticum) medicinal plant: The ethnobiological and ethnomedicinal values of the Marind tribe in Merauke, Papua, Indonesia, Biodiversitas. 17 (2016) 814–822. https://doi.org/10.13057/biodiv/d170259.
Elfahmi, K. Ruslan, S. Batterman, R. Bos, O. Kayser, H.J. Woerdenbag, W.J. Quax, Lignan Profile Of Piper Cubeba, An Indonesian Medicinal Plant, Biochem. Syst. Ecol. 35 (2007) 397–402. Https://Doi.Org/10.1016/J.Bse.2007.01.003.
R.K.B. Zuhrotun, Review Artikel : Potensi Khasiat Obat Tanaman Marga Piper : Piper Nigruml., Piper Retrofractum Vahl., Piper Betle Linn., Piper Cubeba L., Dan Piper Crocatum Ruiz & Pav, J. Farmaka. 16 (2018) 204–212.
C. Suwanphakdee, D.A. Simpson, T.R. Hodkinson, P. Chantaranothai, Typification Of Piper Species (Piperaceae) In Southeast Asia, Especially Thailand, Kew Bull. 73 (2018). Https://Doi.Org/10.1007/S12225-018-9761-6.
W.M.N.H.W. Salleh, F. Ahmad, H.M. Sirat, K.H. Yen, Original Article : Chemical Compositions And Antibacterial Activity Of, Excli J. 11 (2012) 399–406.
E. Rajudin, F. Ahmad, H.M. Sirat, D. Arbain, H.Y. Aboul-Enein, Chemical Constituents From Tiger’s Betel, Piper Porphyrophyllum N.E.Br. (Fam. Piperaceae), Nat. Prod. Res. 24 (2010) 387–390. Https://Doi.Org/10.1080/14786410903421826.
F. Ahmad, Emrizal, H.M. Sirat, F. Jamaludin, N.M. Adah Mustapha, R.M. Ali, D. Arbain, H.Y. Aboul-Enein, Antimicrobial And Anti-Inflammatory Activities Of Piper Porphyrophyllum (Fam. Piperaceae), Arab. J. Chem. 7 (2014) 1031–1033. Https://Doi.Org/10.1016/J.Arabjc.2010.12.032.
A. Arnida, N. Nurlely, N. Kartinah, S. Sutomo, Aktivitas Penghambatan Polimerisasi Hem Ekstrak Etanol Daun Cambai Utan (Piper Porphyrophyllum), Maj. Farmasetika. 4 (2020) 173–177. Https://Doi.Org/10.24198/Mfarmasetika.V4i0.25877.
C. Wiart, N.A. Hannah, M. Yassim, H. Hamimah, M. Sulaiman, Antimicrobial Activity Of Tiger’s Betel (Piper Porphyrophyllum N.E. Br., Piperaceae), Phyther. Res. 18 (2004) 783–784. Https://Doi.Org/10.1002/Ptr.1564.
A.K. Azad, M.A. Jainul, Z.K. Labu, Cytotoxic Activity On Brine Shrimp, Mcf-7 Cell Line And Thrombolytic Potential: Seven Different Medicinal Plant Leaves Extract, J. Sci. Res. 10 (2018) 175–185. Https://Doi.Org/10.3329/Jsr.V10i2.34820.
D. Handayani, M.A. Artasasta, Antibacterial And Cytotoxic Activities Screening Of Symbiotic Fungi Extract Isolated From Marine Sponge Neopetrosia Chaliniformis Ar-01, J. Appl. Pharm. Sci. 7 (2017) 66–69. Https://Doi.Org/10.7324/Japs.2017.70512.
T. Okselni, A. Santoni, A. Dharma, M. Efdi, Biological Activity Of Methanol Extract Of Elaeocarpus Mastersii King: Antioxidant, Antibacterial, And Α-Glucosidase Inhibitor, Rasayan J. Chem. 12 (2019) 146–151. Https://Doi.Org/10.31788/Rjc.2019.1215019.
T. Okselni, A. Santoni, M. Efdi, Isolation Of Secondary Metabolite Compounds From Elaeocarpus Mastersii King Stem Bark And Their Biological Activity Evaluation, Rasayan J. Chem. 15 (2022) 847–852. Https://Doi.Org/10.31788/Rjc.2022.1525294.
M. Efdi, D. Pratama, A. Itam, T. Okselni, Antioxidant Flavonoid Glycoside From Leaves Of Cacao Mistletoe (Scurrula Ferruginea (Jack) Danser), Indones. J. Chem. 22 (2022) 944–952. Https://Doi.Org/10.22146/Ijc.72133.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Jurnal Kimia Unand
This work is licensed under a Creative Commons Attribution 4.0 International License.
Please find the rights and licenses in Jurnal Kimia Unand (J. Kim. Unand). Authors who publish with J. Kim. Unand agree to the following policies. No specific document sign-off is required.
1. License
The use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License.Â
2. Author(s)' Warranties
The author warrants that the article is original, written by the stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons license, the journal permits users to copy, distribute, and display the material. Users will also need to attribute authors and J. Kim. Unand on distributing works in the journal and other media of publications.
4. Rights of Authors
Authors retain all their rights to the published works, such as (but not limited to) the following rights;
- Copyright and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in own future works, including lectures and books,
- The right to reproduce the article for own purposes,
- The right to self-archive the article,
- The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
5. Co-Authorship
Suppose more than one author jointly prepared the article. In that case, any author submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf; and agrees to inform his/her co-authors of the terms of this policy. J. Kim. Unand will not be held liable for anything arising due to the author(s) internal dispute. J. Kim. Unand will only communicate with the corresponding author.
6. Royalties
By submitting the articles, the authors agreed that no fees are payable from J. Kim. Unand.
7. Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, J. Kim. Unand cannot guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.
8. Miscellaneous
J. Kim. Unand will publish the article (or have it published) in the journal if the article’s editorial process is completed and J. Kim. Unand has become obligated to have the article published. The author acknowledges that the article may be published so that it will be publicly accessible, and such access will be free of charge for the readers.