Skrinning Fitokimia, Kandungan Flavonoid Total dan Uji Aktivitas Antioksidan Ekstrak Kayu Balakka (Phyllanthus emblica L.)

Authors

  • Dini Hariyati Adam Fakultas Sains dan Teknologi, Universitas Labuhanbatu

Keywords:

Balakka (Phyllanthus Emblica L.), phytochemicals, activity test, antioxidants, DPPH

Abstract

Balakka plant (Phyllanthus emblica L.) is a plant that has the potential to protect the pancreas, through antioxidant and anti-diabetic activities. This study aims to determine the content of metabolite compounds of balakka wood samples macerated using hexane, ethyl acetate and ethanol solvents, total flavonoid content and antioxidant activity of balakka wood extract. Total flavonoid content was determined using AlCl3 and kursetin standard solution. This AlCl3 method will later produce a red chelate that can be measured with a visible spectrophotometer at a wavelength of 435 nm. While antioxidant activity was measured by the DPPH method. The results showed that the highest total flavonoid content was in the ethanol extract of 60.39 mg/L and antioxidant activity with an IC50 value of 28.97 ppm. Thus, the ethanol extract of balakka wood showed the highest flavonoid content and can be categorized as a very strong antioxidant.

References

Asmara, A.P., Uji Fitokimia Senyawa Metabolit Sekunder Dalam Ekstrak Metanol Bunga Turi Merah (Sesbania grandiflora L. Pers), Al-Kimia. 5 (2017) 48–59. https://doi.org/10.24252/al-kimia.v5i1.2856.

Addina, S &, E.S. Harahap, Analysis of antioxidant activity of balakka barks and fruits ( Phyllanthus emblica) from South Tapanuli, in: IOP Conf Ser Earth Environ Sci, Institute of Physics, 2023: pp. 1–5. https://doi.org/10.1088/1755-1315/1241/1/012098.

Viora, R.H., I. Refi, C.N. Ginting, M.A. Raif, Potential Protective Effects of Balakka Fruit Extract (Phyllanthus emblica L.) Against Doxorubicin-Induced Pancreatic Toxicity in Rats, Jurnal Aisyah : Jurnal Ilmu Kesehatan. 5 (2020) 119–127. https://doi.org/10.30604/jika.v5i1.756.

Bati, K, P.B. Baeti, N.A. Kgakatsi, R.R.T. Majinda, G. Gaobotse, T.E. Kwape, Phytochemical screening, antioxidant, and cytotoxic activities of sequentially extracted Euclea natalensis leaf extracts, Sci Afr. 25 (2024) 2–9. https://doi.org/10.1016/j.sciaf.2024.e02315.

Górniak, Ireneusz, R. Bartoszewski, J. Króliczewski, Comprehensive review of antimicrobial activities of plant flavonoids, Phytochemistry Reviews. 18 (2019) 241–272. https://doi.org/10.1007/s11101-018-9591-z.

Rizki, Utami, Ayudiah, D. Tiara Sitompul, Artikel Review : Stres Oksidatif dan Penyakitnya, (2023). https://www.researchgate.net/publication/366903090.

Rollando, M. Eva, Penetapan Kandungan Fenolik Total Dan Uji Aktivitas Antioksidan Fraksi Air Ekstrak Metanol Kulit Batang Faloak (Sterculia Quadrifida R.Br), Scientia : Jurnal Farmasi Dan Kesehatan. 8 (2018) 29–36. https://doi.org/10.36434/scientia.v8i1.119.

S. Harahap, Alkaloid and Flavonoid Phytochemical Screening on Balakka Leaves (Phyllanthus Emblica L.), Formosa Journal of Science and Technology. 2 (2023) 2069–2082. https://doi.org/10.55927/fjst.v2i8.5691.

Wirasti, Penetapan Kadar Fenolik Total, Flavonoid Total, dan Uji Aktivitas Antioksidan Ekstrak Daun Benalu Petai (Scurrula atropurpurea Dans.) Beserta Penapisan Fitokimia Wirasti, Journal of Pharmaceutical and Medicinal Sciences. 4 (2019) 1–5.

Adlis Santoni, Mai Efdi, N. Fadhillah, Profil Fitokimia dan Penentuan Fenolik Total, Flavonoid Total, dan Uji Aktivitas Antioksidan Ekstrak Daun Sungkai (Peronema canescens Jack) dari Daerah Kota Padang, Jurnal Kimia Unand. 12 (2023) 1–6. https://doi.org/10.25077/jku.12.1.1-6.2023.

N.N.F. Hanin, R. Pratiwi, Kandungan Fenolik, Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Paku Laut (Acrostichum aureum L.) Fertil dan Steril di Kawasan Mangrove Kulon Progo, Yogyakarta, J Trop Biodivers Biotechnol. 2 (2017) 51. https://doi.org/10.22146/jtbb.29819.

E. Prasetyo, N. Zukhruf, W. Kharomah, T. Pudji, R. Program, S. Farmasi, P. Sarjana, S.M. Gombong, J. Kebumen, Uji Aktivitas Antioksidan Menggunakan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Terhadap Ekstrak Etanol Kulit Buah Durian (Durio zibethinnus L.) dari Desa Alasmalang Kabupaten Banyumas, 2021. https://ppjp.ulm.ac.id/journal/index.php/pharmascience.

S. Widodo, N. Made Yusa, P. Timur Ina, Itepa: Jurnal Ilmu dan Teknologi Pangan, Pengaruh Waktu Maserasi Terhadap Aktivitas Antioksidan Ekstrak Daun Mundu (Garcinia dulcis (Roxb.) Kurz) The Influnce Of Maceration Time On Antioxidant Activity Of Mundu Ekstrak Leaf (Garcinia dulcis (Roxb.) Kurz), (n.d.).

M.M.T., A.N.G.L. Lodowik Landi Pote, Pengaruh_Perbedaan_Pelarut_Asam_Pada_Ekstraksi_Ant, Akta Kimindo . 9 (2024) 71–90.

D.H. Adam, Analisis Total Antosianin dari Daun Bayam Merah (Alternanthera Amoena Voss.) Berdasarkan Pengaruh Penambahan Jenis Asam, Edu Science. 2 (2015) 9–12.

B.E. Minarno, SKRINING FITOKIMIA DAN KANDUNGAN TOTAL FLAVANOID PADA BUAH Carica pubescens Lenne &, El-Hayah. 5 (2015) 73–82.

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Published

2024-11-30

How to Cite

Adam, D. H. (2024). Skrinning Fitokimia, Kandungan Flavonoid Total dan Uji Aktivitas Antioksidan Ekstrak Kayu Balakka (Phyllanthus emblica L.). Jurnal Kimia Unand, 13(2), 18–23. Retrieved from http://jku.fmipa.unand.ac.id/index.php/jku/article/view/62

Issue

Vol. 13 No. 2 (2024): November 2024

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Articles

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