Profil Fitokimia dan Penentuan Fenolik Total, Flavonoid Total, dan Uji Aktivitas Antioksidan Ekstrak Daun Sungkai (Peronema canescens Jack) dari Daerah Kota Padang

Authors

  • Adlis Santoni Departemen Kimia FMIPA Universitas Andalas
  • Mai Efdi Departemen Kimia FMIPA Universitas Andalas
  • Nurul Fadhillah Departemen Kimia FMIPA Universitas Andalas

DOI:

https://doi.org/10.25077/jku.12.1.1-6.2023

Keywords:

sungkai (Peronema canescens Jack), maceration, total phenolics, total flavonoids, antioxidants, ABTS

Abstract

Sungkai (Peronema canescens Jack) is a plant that is easy to find in Indonesia. Sungkai leaves are used by the community as herbal medicine to boost immunity. The purpose of this study was to determine the phytochemical profile, total phenolic content and total flavonoids and test the antioxidant activity of Sungkai leaf extract. Sungkai leaf extract was obtained by maceration method in stages. The results of the phytochemical screening showed that ethyl acetate extract and methanol extract contained phenolic and flavonoid compounds. Total phenolic test using the method Folin-Ciocalteu with the highest total phenolic content in methanol extract namely 172.75 mg GAE/g sample. Total flavonoid test using the AlCl3 method with the highest total flavonoid content in the ethyl acetate extract, namely 825.714 mg QE/g sample. Antioxidant activity test using the ABTS method with a very strong antioxidant activity in methanol extract with IC50 of 13.589 mg/L.

References

Ramadenti F,; Sundaryono A,; Handayani D. Uji Fraksi Etil Asetat Daun Peronema canescens terhadap Plasmodium berghei pada Mus musculus. Alotrop J Pendidik dan Ilmu Kim. 2017;2(1):89-92.

Ahmad, I.; Ibrahim, A. Bioaktivitas Ekstrak Metanol dan Fraksi N-Heksana Daun Sungkai (Peronema canescens Jack) Terhadap Larva Udang (Artemia Salina Leach). Jurnal Sains dan Kesehatan. 2015;1(3):114-119.

Artini, P. E. U. D.; Astuti, K. W.; Warditiani, N. K. Uji Fitokimia Ekstrak Etil Asetat Rimpang Bangle (Zingiber purpureum Roxb). Departemen Farmasi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas. 2008;(Iii):1-7.

Fransisca D.; Kahanjak DN.; Frethernety A. Uji aktivitas antibakteri ekstrak etanol daun sungkai ( Peronema canescens Jack ) terhadap pertumbuhan Escherichia coli dengan metode difusi cakram Kirby-Bauer. 2020;4(1):460-470.

Kasumawati F.; Hasnah S. The Effect of Drying Method on Potential Antioxidants in Ethanol Extract of Sungkai Leaf ( Parenoma Canescens Jack .) Simplicia from Kalimantan Pengaruh Pengeringan Simplisia Terhadap Potensi Antioksidan Ekstrak Etanol Daun Sungkai ( Parenoma Canescens Ja. 2022;16(1):1-8.

Santoni A.; Pratama I.; Afrizal. Penentuan Kandungan Metabolit Sekunder, Uji Aktivitas Antibakteri dan Sitotoksik Ekstrak Daun Sungkai (Peronema canescens Jack). Jurnal Kimia Unand. 2020, 9(4), 21-25.

Kitagawa, I.; Simanjuntak, P.; Hori, K.; Nagami, N.; Mahmud, T.; Shibuya, H.; Kobayashi, M. Seven New Clerodane-Type Diterpenoids, Peronemins A2, A3, B1, B2, B3, C1, And D1, from the Leaves Of Peronema canescens (Verbenaceae). Journal Chemical Pharmacy Bulletin, 1994, 42 (5), 1050–1055.

Pindan N. P.; Saleh C.; Magdaleni A. R. Uji Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Fraksi N-Heksana, Etil Asetat dan Etanol sisa dari Daun Sungkai (Peronema canescens Jack) dengan Metode DPPH. Jurnal Atomik. 2021, 06(1), 22-27.

Obenu, N. M. Ekstraksi dan Identifikasi Kandungan Metabolit Fraksi Diklorometana dan Akuades Ekstrak Metanol Daun Sirsak (Annona muricata Linn). Jurnal Saintek Lahan Kering. 2019;2(1):17-19.

Shalaby, E. A.; Shanab, S. M. M. Comparison of DPPH and ABTS Assays for Determining Antioxidant Potential of Water and Methanol Extracts of Spirulina platensis. Indian J. Mar. Sci.2013, 42(5).

Molyneux, P. The Use of the Stable Free Radical Diphenylpicryl-Hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 2004, 26 (December 2003).

Downloads

Published

2023-05-30

How to Cite

Adlis Santoni, Mai Efdi, & Fadhillah, N. (2023). Profil Fitokimia dan Penentuan Fenolik Total, Flavonoid Total, dan Uji Aktivitas Antioksidan Ekstrak Daun Sungkai (Peronema canescens Jack) dari Daerah Kota Padang. Jurnal Kimia Unand, 12(1), 1–6. https://doi.org/10.25077/jku.12.1.1-6.2023

Issue

Vol. 12 No. 1 (2023): May 2023

Section

Articles

License

Copyright (c) 2023 Jurnal Kimia Unand

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Please find the rights and licenses in Jurnal Kimia Unand (J. Kim. Unand).  Authors who publish with J. Kim. Unand agrees to the following policies. No specific document sign-off is required.

1. License

The use of the article will be governed by the Creative Commons Attribution license as currently displayed on the Creative Commons Attribution 4.0 International License.

2. Author(s)' Warranties

The author warrants that the article is original, written by the stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights

Under the Creative Commons license, the journal permits users to copy, distribute, and display the material. Users will also need to attribute authors and J. Kim. Unand on distributing works in the journal and other media of publications.

4. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

  • Copyright and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in own future works, including lectures and books,
  • The right to reproduce the article for own purposes,
  • The right to self-archive the article,
  • The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

5. Co-Authorship

Suppose more than one author jointly prepared the article. In that case, any author submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf; and agrees to inform his/her co-authors of the terms of this policy. J. Kim. Unand will not be held liable for anything arising due to the author(s) internal dispute. J. Kim. Unand will only communicate with the corresponding author.

6. Royalties

By submitting the articles, the authors agreed that no fees are payable from J. Kim. Unand.

7. Privacy Statement 

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.

However, due to the advancement and availability of hacking and data mining techniques found over the Internet,  J. Kim. Unand cannot guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.

8. Miscellaneous

J. Kim. Unand will publish the article (or have it published) in the journal if the article’s editorial process is completed and J. Kim. Unand has become obligated to have the article published. The author acknowledges that the article may be published so that it will be publicly accessible, and such access will be free of charge for the readers.